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Fast Probe U Lyo qPCR Mastermix

57 - 385 *Net price

Ready-to-use mastermix for qPCR with fluorescent probes, additionally contains UDG to prevent carryover contaminations. Supplied in lyophilized form.

Product code: M-FPU-L Categories: ,
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Description

Description

Fast Probe U Lyo qPCR Mastermix is a universal, ready-to-use reagent for quantitative and/or qualitative PCR with real-time detection. The mastermix is optimized for DNA/LNA dual-labeled fluorescent probes. Supplied in lyophilized form. The mastermix contains a hot-start Taq DNA polymerase, which is activated automatically during the initial DNA denaturation step. This prevents the extension of non-specifically annealed primers and the formation of primer-dimers at low temperatures during qPCR setup. Special additives increase its resistance to common PCR inhibitors. Presence of uracil-DNA-glycosylase and dUTP helps to prevent carryover contamination. The mastermix provides reproducible results with high sensitivity, enabling the detection of single copies of a DNA target.

Properties

Benefits

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance
  • Prevention of false-positive results
  • Storage at RT

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual-labelled probes

Storage and transportation conditions:

  • RT (< +25°С) – 1 year

Documents

Certificate of Analysis (CoA)