Diamant TaqA DNA Polymerase

110 - 1 300

Hot-start Taq DNA polymerase with activation temperature above 37°C.
Supplied with 10x PCR buffer and 50mM MgCl2.

Product code: E-TAP Category:
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Description

Diamant TaqA DNA Polymerase is a high-quality hot-start polymerase with superior sensitivity and specificity compared to native Taq polymerase. The reagent is composed of high-purified recombinant Taq DNA polymerase and specific low-molecular weight aptamer which inhibits enzymatic activity of polymerase at room temperature. According to our tests, the polymerase keeps inactive during the incubation with a substrate for about 1 hour at 37°C. Activation of the enzyme starts automatically during the first cycle of DNA denaturation. Due to original storage buffer the reagent can be stored at +2°С – +8°С for 6 months without significant changes in activity and inactivation.

Benefits:

  • Amplification of PCR fragments up to 5 000 bp
  • Stable at +2°С – +8°С up to 6 month

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual-labelled probes
  • PCR with intercalating dyes

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

  • 5 units/μl (5-100 units/μl upon request)

Storage and transportation conditions:

  • From -24°С to -16°С – 3 years
  • From +2°С to +8°С – 6 months
  • Up to 30 days at a temperature < +25°С

Additional information

Product Size

1 000 U/0.2 ml
2 500 U/0.5 ml
25 000 U/5×1 ml
5 000 U/1 ml
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