RevM Hot Start Revertase

90 - 715

MMLV Reverse Transcriptase with hot-start.
Supplied with 10X reverse transcription buffer.

Product code: E-RTH Category:
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Description

RevM Hot Start Revertase is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV), is intended for the synthesis of a complementary DNA strand (cDNA) on a single-stranded RNA template. Due to the presence of several mutations the enzyme possesses inhibited RNase H activity and has increased thermostability. The enzyme possesses reduced activity at temperatures below 37°C and has an optimal working temperature of 55°C. Inactivation occurs after 10 minutes at 80°C. Compared to wild-type MMLV reverse transcriptase RevM provides a higher yield of cDNA, has improved efficiency on GC-rich RNA templates and also has a higher productivity.

Benefits:

  • Effective for GC-rich RNA templates
  • Reduced activity at temperatures below 37°С
  • Optimum working temperature is 55°С
  • High productivity

Area of applications:

  • One-step RT-PCR

Storage and dilution buffer:

  • 20 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 100 mM KCl, 50% glycerol, 0.5% Tween-20, 1 mM TCEP

Concentration:

  • 5 units/μl (5-100 units/μl upon request)

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 14 days at a temperature < +4°С

Additional information

Product Size

25 000 U/5×1 ml
5 000 U/1 ml
50 000 U/10 ml
Free sample