RevM Hot-Start Revertase

Description

RevM Hot-Start Revertase is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV), is intended for the synthesis of a complementary DNA strand (cDNA) on a single-stranded RNA template. Due to the presence of several mutations the enzyme possesses inhibited RNase H activity and has increased thermostability. The enzyme possesses reduced activity at temperatures below 37°C and has an optimal working temperature of 55°C. Inactivation occurs after 10 minutes at 80°C. Compared to wild-type MMLV reverse transcriptase RevM provides a higher yield of cDNA, has improved efficiency on GC-rich RNA templates and also has a higher productivity. Supplied in glycerol-free buffer.

Benefits:

• Effective for GC-rich RNA templates
• Reduced activity at temperatures below 37°С
• Optimum working temperature is 55°С
• High productivity

Area of applications:

• Multiplex RT-PCR
• Routine RT-PCR
• Low copy RT-PCR
• RT-PCR with dual-labelled probes

Concentration:

• 10 units/μl

Storage and dilution buffer:

• 20 mM Tris HCl (pH 7.5), 1 mM EDTA, 100 mM KCl, 750 mM Trehalose, 5 mM TCEP

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From + 2°С to + 8°С – 14 days
• 10 freeze/thaw cycles are allowed