Uracil-DNA Glycosylase
90€ - 370€ *Net price
Enzyme for prevention of false-positive results in PCR.
* GF โ supplied in glycerol-free buffer.
- Description
- Properties
- Additional information
- Documents
Description
Uracil-DNA Glycosylase (UNDG) is a 25.5 KDa protein isolated from a recombinant E. coli strain bearing the ung gene of the E. coli str. K-12. It hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA, creating alkali-sensitive abasic sites. These abasic sites can be hydrolyzed by endonuclease, heat, or alkali treatment.
The enzyme is highly purified by affinity and anion-exchange chromatography. UNDG can be supplied in glycerin and detergent free buffer for the subsequent lyophilization upon request. The enzyme is inactivated completely during the first cycle of DNA denaturation and does not interfere with the current reaction amplification products.
Benefits:
- Prevents false-positive results
- Is inactivated during the first PCR cycle
- Maximal activity at 37ยฐC
Area of applications:
- Routine PCR
- Multiplex PCR
- Low copy PCR
- PCR with dual-labelled probes
- PCR with intercalating dyes
Concentration:
- 5 units/ฮผl (500 units/ฮผl for glycerol-free enzyme)
Storage and transportation conditions:
- From -24ยฐะก to -16ยฐะก โ 1 year
- From +2ยฐะก to +8ยฐะก โ 14 days
Additional information
| Product Size | 5 000 U/0.01 ml/GF |
|---|
