Diamant Taq DNA Polymerase

Description

Diamant Taq DNA Polymerase is a 94 KDa thermostable protein isolated from a recombinant E. coli strain bearing the polA gene of the Thermus aquaticus YT1 polymerase. The enzyme catalyses’ synthesis (replication) of DNA chain in direction from 5’ to 3’ end and does not perform corrective 3’-5’ exonuclease activity. In addition, this enzyme possesses deoxynucleotidyl transferase activity (TdT), which leads to the connection of an additional residue dA at the 3’ end of the PCR product. The enzyme is highly purified by affinity and anion-exchange chromatography. Diamant Taq DNA Polymerase can be supplied in glycerol and detergent free buffer for the subsequent lyophilization
upon request.

Benefits:

• Amplification of PCR fragments up to 5 000 bp

Area of applications:

• Routine PCR

Storage and dilution buffer:

• 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

• from 5 to 1000 units/μl

Storage and transportation conditions:

• From -24°С to -16°С – 3 years
• Up to 30 days at a temperature < +25°С

Additional information