5X Fast Probe U Lyo-Ready qPCR Mastermix
125€ - 1 315€ *Net price
Ready-to-use mastermix for qPCR with fluorescent probes, additionally contains UDG to prevent carryover contaminations. Supplied in lyo-ready form.
Description
Description
5X Fast Probe U Lyo-Ready qPCR Mastermix is a universal, ready-to-use reagent for quantitative and/or qualitative PCR with real-time detection. The mastermix is optimized for DNA/LNA dual-labeled fluorescent probes. Supplied in lyo-ready form. The mastermix contains a hot-start Taq DNA polymerase, which is activated automatically during the initial DNA denaturation step. This prevents the extension of non-specifically annealed primers and the formation of primer-dimers at low temperatures during qPCR setup. Special additives increase its resistance to common PCR inhibitors. Presence of uracil-DNA-glycosylase and dUTP helps to prevent carryover contamination. The mastermix provides reproducible results with high sensitivity, enabling the detection of single copies of a DNA target.
Properties
Benefits
- High sensitivity (single copies of DNA)
- Increased inhibition resistance
- Prevention of false-positive results
- Ready for lyophilization
Area of applications:
- Multiplex PCR
- Routine PCR
- Low copy PCR
- PCR with dual-labelled probes
Storage and transportation conditions:
- From -24°С to -16°С – 1 year
- From +2°С to +8°С – 14 days
- 10 freeze/thaw cycles are allowed
