Diamant TaqF DNA Polymerase

135 - 1 595

Hot-start Taq DNA polymerase with activation temperature above 90°C.
Supplied with 10x PCR buffer and 50mM MgCl2.

Product code: E-TFP Category:
Clear
View cart
Request for bulk size

Description

Diamant TaqF DNA Polymerase is a chemically modified Taq DNA polymerase. Diamant TaqF DNA Polymerase has a high inactivation level and shows no activity after its incubation for 1 hour at 60°С. Enzyme is activated by 15 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during the PCR setup. The enzyme catalyses the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction and also exhibits 5’ exonuclease activity.

Benefits:

  • High inactivation level
  • High activity at the end of PCR

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual-labelled probes
  • PCR with intercalating dyes

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

  • 5 units/μl (5-20 units/μl upon request)

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 14 days at a temperature < +4°С

Additional information

Product Size

1 000 U/0.2 ml
2 500 U/0.5 ml
25 000 U/5×1 ml
5 000 U/1 ml
Free sample