Blitz DNA Polymerase

180 - 1 360

Chimeric polymerase with increased accuracy and resistance to inhibitors. Suitable for long range PCR and preparation of NGS libraries.
Supplied with 2.5X PCR buffer.

Product code: E-BLP Category:
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Description

Blitz DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The modifications of amino acid structure of the native Pfu results in shorter extension times (20 s/kb), more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Blitz a superior choice for cloning. This enzyme is suitable for all PCR applications requiring greater accuracy or long amplicons. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction and also exhibits 3’→5’ exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5’ exonuclease activity.

Benefits:

  • Increased DNA synthesis rate (up to 3 000 bp/min)
  • Long fragments amplification (10 kbp or longer)
  • Increased inhibition resistance

Area of applications:

  • PCR with increased synthesis accuracy
  • Amplification of long DNA fragments
  • Direct PCR without samples purifications
  • NGS libraries preparation

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

  • 2 units/ΞΌl

Storage and transportation conditions:

  • From -24Β°Π‘ to -16Β°Π‘ – 2 years
  • Up to 7 days at a temperature < +25Β°Π‘

Additional information

Product Size

1 000 U/0.5 ml
2 500 U/1.25 ml
5 000 U/2×1.25 ml
500 U/0.25 ml
Free sample