Blitz DNA Polymerase

180 - 1 360 *Net price

Chimeric polymerase with increased accuracy and resistance to inhibitors. Suitable for long range PCR and preparation of NGS libraries.
Supplied with 2.5X PCR buffer.

Product code: E-BLP Category:
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Blitz DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The modifications of amino acid structure of the native Pfu results in shorter extension times (20 s/kb), more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Blitz a superior choice for cloning. This enzyme is suitable for all PCR applications requiring greater accuracy or long amplicons. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction and also exhibits 3’→5’ exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5’ exonuclease activity.


  • Increased DNA synthesis rate (up to 3 000 bp/min)
  • Long fragments amplification (10 kbp or longer)
  • Increased inhibition resistance

Area of applications:

  • PCR with increased synthesis accuracy
  • Amplification of long DNA fragments
  • Direct PCR without samples purifications
  • NGS libraries preparation

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20


  • 2 units/μl

Storage and transportation conditions:

  • From -24°С to -16°С – 1 year
  • From +2°С to +8°С – 14 days

Additional information

Product Size

5 000 U/2×1.25 ml/B
2 500 U/1.25 ml/B
1 000 U/0.5 ml/B
500 U/0.25 ml/B
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Certificate of Analysis