Blitz DNA Polymerase
180€ - 1 360€ *Net price
Chimeric polymerase with increased accuracy and resistance to inhibitors. Suitable for long range PCR and preparation of NGS libraries.
Supplied with 2.5X PCR buffer.
- Description
- Properties
- Additional information
- Documents
Description
Blitz DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The modifications of amino acid structure of the native Pfu results in shorter extension times (20 s/kb), more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Blitz a superior choice for cloning. This enzyme is suitable for all PCR applications requiring greater accuracy or long amplicons. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5ββ3β direction and also exhibits 3ββ5β exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5β exonuclease activity.
Benefits:
- Increased DNA synthesis rate (up to 3 000 bp/min)
- Long fragments amplification (10 kbp or longer)
- Increased inhibition resistance
Area of applications:
- PCR with increased synthesis accuracy
- Amplification of long DNA fragments
- Direct PCR without samples purifications
- NGS libraries preparation
Storage and dilution buffer:
- 20 mΠ Tris-HCl (pH 8.0), 0.5 mΠ EDTA, 100 mΠ KCl, 50% glycerol, 0.5% Tween-20
Concentration:
- 2 units/ΞΌl
Storage and transportation conditions:
- From -24Β°Π‘ to -16Β°Π‘ β 1 year
- From +2Β°Π‘ to +8Β°Π‘ β 14 days
Additional information
| Product Size | 1 000 U/0.5 ml |
|---|
