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FloraCheck BV PCR kit

321 *Net price

FloraCheck BV PCR Kit is designed for the qPCR-based detection of bacterial vaginosis caused by vaginal microbiome dysbiosis, specifically evaluating the balance between normally predominant Lactobacillus spp. and opportunistic pathogens Gardnerella vaginalis and Atopobium vaginae

Product code: FC-BV-100 Categories: ,
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Description

Description

FloraCheck BV PCR Kit is designed to detect changes in the vaginal microbiome caused by an imbalance between the concentration of normally predominant Lactobacillus spp. and opportun Lactobacillus sppistic bacteria Gardnerella vaginalis and Atopobium vaginae, which can lead to infectious inflammation. The kit also determines total bacterial DNA in biological material (swabs, scrapings, and vaginal discharge) using real-time quantitative PCR. The limit of detection (LoD) is 1 000 copies/ml for Lactobacillus spp., G. vaginalis, or A. vaginae, based on extraction from a 100 µL sample volume. To prevent false-positive results caused by carryover contamination from previous amplification products, the kit includes uracil-DNA glycosylase (UDG) and dUTP.

Properties

Benefits:

  • High specificity (up to 99.9%)
  • Limit of detection – 500 copies/ml
  • •Contains UDG and dUTP eliminating carryover contamination

Detection pathogen:

  • Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp.

Area of Applications:

  • Bacterial vaginosis (BV) diagnostics
  • Urogenital microbiota analysis

Storage and transportation conditions:

  • From -24°С to -16°C – 1 year
  • From +2°С to +8°C – 14 days
  • 10 freeze/thaw cycles are allowed

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