New kit for detection of bacterial vaginosis using qPCR
FloraCheck BV PCR Kit uses real-time quantitative PCR to detect bacterial vaginosis by monitoring changes in the vaginal microbiome. It accurately measures the imbalance between predominant Lactobacillus spp. and opportunistic bacteria (Gardnerella vaginalis and Atopobium vaginae) in biological samples, including vaginal smears, scrapings, and discharge.
Example of PCR efficiency for the FloraCheck BV kit in bacterial vaginosis diagnostics
Figure 1. Results of calibrators (synthetic DNA) amplification with FloraCheck BV PCR Kit. Blue – bacterial DNA, green – G. vaginalis, orange – Lactobacillus spp., violet – A. vaginae.
Figure 2. Results of BV- (negative control) amplification with FloraCheck BV PCR Kit. Blue – bacterial DNA, green – G. vaginalis, orange – Lactobacillus spp., violet – A. vaginae. The final diagnosis could be made by comparing the concentrations of total bacterial mass, lactobacilli and potential pathogens of vaginosis.
Figure 3. Results of BV+ (positive control) amplification with FloraCheck BV PCR Kit. Blue – bacterial DNA, green – G. vaginalis, orange – Lactobacillus spp., violet – A. vaginae. The final diagnosis could be made by comparing the concentrations of total bacterial mass, lactobacilli and potential pathogens of vaginosis.