5X Direct One-Step RT-PCR Mastermix

Description

5Х Direct One-Step RT-PCR Mastermix is a ready-to-use universal reagent for the synthesis of cDNA on RNA matrix with the following polymerase chain reaction with real time identification of products (RT-PCR). Synthesis of cDNA and qPCR assay is carried out in one tube. Clinical samples (in a saline or PBS transport media) can be used directly for subsequent amplification.
The reagent provides reproducible results with high sensitivity (single copies of RNA) in RT-PCR. Mastermix is recomended for direct SARS-CoV 2 detection. 5X Direct One-Step RT-PCR Mastermix contains all the necessary components for PCR assay, including: “warm-start” reverse transcriptase, hot-start thermostable DNA polymerase and optimized buffer. dNTP mix (25 mM each) supplied in separate tube. Due to some genetic modifications of MMLV reverse transcriptase has inhibited RNase H activity, improved thermostability and increased productivity with GC-rich RNA matrix, resulting in the higher cDNA yield. Hot-start inactivated DNA polymerase of the mastermix does not possess any enzymatic activity during PCR mix preparation and reverse transcription that lead to significant increase of sensitivity and specificity of the assay. Special additives provide increased resistance to inhibitors and allow to use clinical samples without preliminary purification.

Benefits

• High sensitivity (single copies of RNA)
• Synthesis of cDNA and PCR is carried out in one tube
• Increased inhibition resistance

Area of applications:

• Multiplex RT-PCR
• Routine RT-PCR
• Low copy RT-PCR
• RT-PCR with dual-labelled probes

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From +2°С to +8°С – 7 days
• 10 freeze/thaw cycles are allowed

Additional information