5X Fast Probe qPCR U Mastermix

Description

5Х Fast Probe qPCR U Mastermix is a ready-to-use reagent for real-time polymerase chain reaction (qPCR) with fluorescent probes. The reagent contains the following components: thermostable hot-start DNA polymerase, dNTP (including dUTP), uracil-DNA glycosylase (UDG), optimized buffer for qPCR. The using of inactivated DNA polymerase, which does not possess enzymatic activity under the conditions of PCR mixture preparation, leads to an increase in the specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first cycle of DNA denaturation.

The presence of dUTP in the reaction mixture leads to the fact that all amplification products will contain uracil. In this case, UDG effectively hydrolyzes uracil from single- and double-stranded DNA molecules during the preparation of the reaction, thus preventing the amplification of the products of previous PCR (amplicons). It helps to avoid false-positive results. UDG is completely inactivated during PCR and does not interfere with the amplification of the products of the current reaction. Special additives provide increased resistance to inhibitors. Mastermix can also contain reference dye ROX (final concentration is 0.5 μM (High ROX) or 0.03 μM (Low ROX) depending on qPCR instrument).

Benefits

• High sensitivity (single copies of DNA)
• Increased inhibition resistance
• Prevention of false-positive results

Area of applications:

• Multiplex PCR
• Routine PCR
• Low copy PCR
• PCR with dual-labelled probes

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From  +2°С to +8°С – 14 days
• 10 freeze/thaw cycles are allowed

Additional information