5X Fast Probe qPCR Mastermix

95 - 995 *Net price

Ready-to-use mastermix for qPCR with fluorescent probes.

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Description

5X Fast Probe qPCR Mastermix is a universal, ready-to-use reagent for quantitative and/or qualitative PCR with real-time detection. The mastermix is optimized for DNA/LNA dual-labeled fluorescent probes.

The mastermix contains a hot-start Taq DNA polymerase, which is activated automatically during the initial DNA denaturation step. This prevents the extension of non-specifically annealed primers and the formation of primer-dimers at low temperatures during qPCR setup. Special additives increase its resistance to common PCR inhibitors. The mastermix provides reproducible results with high sensitivity, enabling the detection of single copies of a DNA target.

Benefits

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual-labelled probes

Storage and transportation conditions:

  • From -24°С to -16°С – 1 year
  • From +2°С to +8°С – 14 days
  • 10 freeze/thaw cycles are allowed

Additional information

Product options

5 000 reactions/20×1.25 ml
1 000 reactions/4×1.25 ml
500 reactions/2×1.25 ml
250 reactions/1×1.25 ml
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