2Х Fast Dye UR qPCR Mastermix
110€ - 1 155€ *Net price
Ready-to-use mastermix for qPCR with intercalating dye, additionally contains UDG to prevent carryover contamination. Supplied with ROX reference dye.
- Description
- Properties
- Additional information
- Documents
Description
2X Fast Dye UR qPCR Mastermix is a universal, ready-to-use reagent for quantitative and/or qualitative PCR with with intercalating dye.
The mastermix contains a hot-start Taq DNA polymerase, which is activated automatically during the initial DNA denaturation step. This prevents the extension of non-specifically annealed primers and the formation of primer-dimers at low temperatures during qPCR setup. Special additives increase its resistance to common PCR inhibitors. Presence of uracil-DNA-glycosylase and dUTP helps to prevent carryover contamination. The mastermix provides reproducible results with high sensitivity, enabling the detection of single copies of a DNA target.
The Mastermix supplied with ROX reference dye (final concentration in reaction mixture is 0.5 μM (High ROX) or 0.05 μM (Low ROX) depending of qPCR instrument). The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
Benefits:
- High sensitivity (single copies of DNA)
- Increased inhibition resistance
- Prevention of false-positive results
Area of applications:
- PCR with intercalating dye
Storage and transportation conditions:
- From -24°С to -16°С – 1 year
- From +2°C to +8°C – 14 days
- 10 freeze/thaw cycles are allowed
Additional information
| Product options | 5 000 reactions/50 ml |
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