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2X Fast Dye U qPCR Mastermix

105 - 1 100 *Net price

Ready-to-use mastermix for qPCR with intercalating dye additionally contains UDG to prevent carryover contamination.

Product code: M2-FDU Categories: ,
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Description

2X Fast Dye U qPCR Mastermix is a universal, ready-to-use reagent for quantitative and/or qualitative PCR with with intercalating dye. The mastermix contains a hot-start Taq DNA polymerase, which is activated automatically during the initial DNA denaturation step. This prevents the extension of non-specifically annealed primers and the formation of primer-dimers at low temperatures during qPCR setup. Special additives increase its resistance to common PCR inhibitors. Presence of uracil-DNA-glycosylase and dUTP helps to prevent carryover contamination. The mastermix provides reproducible results with high sensitivity, enabling the detection of single copies of a DNA target.

Benefits:

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance
  • Prevention of false-positive results

Area of applications:

  • PCR with intercalating dye

Storage and transportation conditions:

  • From -24°С to -16°С – 1 year
  • From +2°C to +8°C – 14 days
  • 10 freeze/thaw cycles are allowed

Additional information

Product options

5 000 reactions/50 ml
1 000 reactions/8×1.25 ml
500 reactions/4×1.25 ml
250 reactions/2×1.25 ml
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