2X Blitz Mastermix
170€ - 1 040€ *Net price
Ready-to-use mastermix for PCR with increased synthesis accuracy and resistance to inhibitors. Suitable for preparation of NGS libraries.
- Description
- Properties
- Additional information
- Documents
Description
2X Blitz Mastermix is a ready-to-use mixture for polymerase chain reaction (PCR) with increased accuracy of DNA synthesis. Mastermix contains all the necessary components for PCR, including: thermostable DNA polymerase, dNTP and optimized buffer. The DNA polymerase included in the Blitz mastermix is a chimeric thermostable protein consisting of Pfu polymerase and SSO7d DNA-binding domain. The DNA-binding domain stabilizes the complex of DNA polymerase with the template, it leads to an increase in the processivity, synthesis rate, accuracy and stability of the enzyme in a high ionic strength of the solution. The mastermix has an increased rate of DNA synthesis (up to 3 000 bp/min) and is capable of amplifying DNA fragments longer than 10 000 bp.
Benefits:
- Increased DNA synthesis rate (up to 3 000 bp/min)
- Long fragments amplification (10 kbp or longer)
- Increased inhibition resistance
Area of applications:
- PCR with increased synthesis accuracy
- Amplification of long DNA fragments
- NGS libraries preparation
- Direct PCR without samples purifications
Storage and transportation conditions:
- From -24°С to -16°С – 1 year
- From +2°С to +8°С – 14 days
- 10 freeze/thaw cycles are allowed
Additional information
| Product Size | 800 reactions/8×1.25 ml |
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