5X Fast Probe qPCR Mastermix

Description

5Х Fast Probe qPCR Mastermix is a universal ready-to-use reagent for quantitative and/or qualitive PCR with real-time detection of the results. The qPCR Mix contains optimized components and inactivated DNA polymerase supplied in a proprietary reaction buffer. 5Х Fast Probe qPCR Mastermix is optimized for DNA/LNA hydrolysis probes based on the 5 ́-3 ́ exonuclease activity. DNA Polymerase is activated by 2 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. 5X Fast Probe qPCR Mastermix consists of the following components:
– thermostable DNA polymerase with a hot-start;
– dNTP;
– optionally ROX reference dye (final concentration is 0.5 μM (High ROX) or 0.03 μM (Low ROX) depending of qPCR instrument);
– MgCl2 (final concentration is 5 mM);
– optimized buffer.

Benefits

• High sensitivity (single copies of DNA)
• Increased inhibition resistance

Area of applications:

• Multiplex PCR
• Routine PCR
• Low copy PCR
• PCR with dual-labelled probes

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From +2°С to +8°С – 14 days
• 10 freeze/thaw cycles are allowed

Additional information