5X Fast Probe qPCR Mastermix

95 - 995

Ready-to-use mastermix for qPCR with fluorescent probes.

Product code: M5-FP Category:
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Description

5Х Fast Probe qPCR Mastermix is a universal ready-to-use reagent for quantitative and/or qualitive PCR with real-time detection of the results. The qPCR Mix contains optimized components and inactivated DNA polymerase supplied in a proprietary reaction buffer. 5Х Fast Probe qPCR Mastermix is optimized for DNA/LNA hydrolysis probes based on the 5 ́-3 ́ exonuclease activity. DNA Polymerase is activated by 2 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. 5X Fast Probe qPCR Mastermix consists of the following components:
– thermostable DNA polymerase with a hot-start;
– dNTP;
– optionally ROX reference dye (final concentration is 0.5 μM (High ROX) or 0.03 μM (Low ROX) depending of qPCR instrument);
– MgCl2 (final concentration is 5 mM);
– optimized buffer.

Benefits

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance
  • Stable at temperatures of +2°C – +8°C

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual-labelled probes

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • From + 2°С to + 8°С – 12 months
  • Up to 30 days at a temperature < +25°С
  • 20 freeze/thaw cycles are allowed

Additional information

Product Size

250 reactions/1×1.25 ml
500 reactions/2×1.25 ml
1 000 reactions/4×1.25 ml
5 000 reactions/20×1.25 ml
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