5X Fast Dye qPCR U Mastermix

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Ready-to-use mastermix for qPCR with intercalating dyes, additionally contains UDG to prevent false-positives results.

Product code: M5-FDU Category:
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Description

5Х Fast Dye qPCR U Mastermix is a versatile, ready-to-use reagent for real-time polymerase chain reaction with intercalating dye. The solution contains substances that increase half-life and processivity of Taq DNA polymerase by enhancing its stability during PCR. Presence of dUTP in the reaction mixture leads to the fact that all the amplification products will contain uracil in their composition. In this case, UDG in the new PCR run effectively hydrolyses uracil from single- and double-stranded DNA molecules during the reaction setup, thus preventing carry-over contamination by amplicon of preceding PCR and obtaining false-positive results. UDG is inactivated during the first DNA denaturation cycle and does not interfere with the amplification of the products of the current reaction.

5X Fast Dye qPCR U Mastermix contains DNA polymerase inactivated by antibodies, which does not exhibit enzymatic activity during reaction mixture preparation, which leads to increasing of reaction specificity and sensitivity. Enzyme activation occurs automatically during the first cycle of DNA denaturation. Optionally Mastermix can contains ROX reference dye (final concentration is 0.5 μM (High ROX) or 0.03 μM (Low ROX) depending of qPCR instrument).

Benefits

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance
  • Prevention of false-positive results

Area of applications:

  • PCR with intercalating dye

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 14 days at a temperature < +25°С
  • 10 freeze/thaw cycles are allowed

Additional information

Product Size

250 reactions/1×1.25 ml
500 reactions/2×1.25 ml
1 000 reactions/4×1.25 ml
5 000 reactions/20×1.25 ml
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