2X Blitz Mastermix

170 - 1 040

Ready-to-use mastermix for PCR with increased synthesis accuracy and resistance to inhibitors. Suitable for preparation of NGS libraries.

Product code: M2-BL Category:
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Description

2X Blitz Mastermix is a ready-to-use mixture for polymerase chain reaction (PCR) with increased accuracy of DNA synthesis. Mastermix contains all the necessary components for PCR, including: thermostable DNA polymerase, dNTP and optimized buffer. The DNA polymerase included in the Blitz mastermix is a chimeric thermostable protein consisting of Pfu polymerase and SSO7d DNA-binding domain. The DNA-binding domain stabilizes the complex of DNA polymerase with the template, that leads to an increase in the processivity, synthesis rate, accuracy and stability of the enzyme in a high ionic strength of the solution. The mastermix has an increased rate of DNA synthesis (up to 3 000 bp/min) and is capable of amplifying DNA fragments longer than 10 000 bp.

Benefits:

  • Increased DNA synthesis rate (up to 3 000 bp/min)
  • Long fragments amplification (10 kbp or longer)
  • Increased inhibition resistance

Area of applications:

  • PCR with increased synthesis accuracy
  • Amplification of long DNA fragments
  • NGS libraries preparation

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 7 days at a temperature < +25°С
  • 10 freeze/thaw cycles are allowed

Additional information

Product Size

100 reactions/1×1.25 ml
200 reactions/2×1.25 ml
400 reactions/4×1.25 ml
800 reactions/8×1.25 ml
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