Thermolabile Uracil-DNA Glycosylase
90€ - 720€ *Net price
Enzyme for prevention of false-positive results in PCR, RT-PCR and LAMP.
* GF – supplied in glycerol-free buffer.
- Description
- Properties
- Additional information
- Documents
Description
Thermolabile Uracil-DNA Glycosylase (tUDG) is a 25.5 KDa protein isolated from a recombinant E. coli strain bearing cloned UNDG gene from a psychrophilic marine bacterium. It hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA, creating alkali-sensitive abasic sites. These abasic sites can be hydrolyzed by endonuclease, heat, or alkali treatment. The enzyme is used in the preparation of PCR, RT-PCR and LAMP mixtures to prevent carryover contamination.
The enzyme is highly purified by affinity and anion-exchange chromatography. The enzyme is inactivated completely during 5 minutes at 55°С and does not interfere with the current reaction amplification products.
Benefits:
- Prevents carryover contamination
- Inactivated at 55°С
Area of applications:
- Routine PCR
- Routine RT-PCR
- LAMP
Concentration:
- 5 units/μl (500 units/μl for glycerol-free enzyme)
Storage and dilution buffer:
- 20 mM Tris-HCl (pH 7.0), 0.5 mM EDTA, 100 mM KCl, 50% glycerol, 0.1 mg/ml BSA
Storage and transportation conditions:
- From -24°С to -16°С – 1 year
Additional information
| Product Size | 5 000 U/0.01 ml/GF |
|---|
