Thermolabile Uracil-DNA Glycosylase

90 - 720 *Net price

Enzyme for prevention of false-positive results in PCR, RT-PCR and LAMP.
* GF – supplied in glycerol-free buffer.

Product code: E-TUDG Category:
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Thermolabile Uracil-DNA Glycosylase (tUDG) is a 25.5 KDa protein isolated from a recombinant E. coli strain bearing cloned UNDG gene from a psychrophilic marine bacterium. It hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA, creating alkali-sensitive abasic sites. These abasic sites can be hydrolyzed by endonuclease, heat, or alkali treatment. The enzyme is used in the preparation of PCR, RT-PCR and LAMP mixtures to prevent carryover contamination.
The enzyme is highly purified by affinity and anion-exchange chromatography. The enzyme is inactivated completely during 5 minutes at 55°С and does not interfere with the current reaction amplification products.


  • Prevents carryover contamination
  • Inactivated at 55°С

Area of applications:

  • Routine PCR
  • Routine RT-PCR
  • LAMP


  • 5 units/μl (500 units/μl for glycerol-free enzyme)

Storage and dilution buffer:

  • 20 mM Tris-HCl (pH 7.0), 0.5 mM EDTA, 100 mM KCl, 50% glycerol, 0.1 mg/ml BSA

Storage and transportation conditions:

  • From -24°С to -16°С – 1 year

Additional information

Product Size

5 000 U/0.01 ml/GF
5×500 U/5×0.1 ml
500 U/0.1 ml
10×500 U/10×0.1 ml
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