RevM Revertase

80 - 150

MMLV Reverse Transcriptase.
Supplied with 10X reverse transcription buffer.

Product code: E-RT Category:
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Description

RevM Revertase is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV) which performs the synthesis of a complementary strand of DNA (cDNA) on a single-stranded RNA template. Due to the presence of several mutations, the enzyme possesses inhibited RNase H activity and has increased thermostability at below 65°C, with an optimal temperature of 55°C. Compared to wild-type MMLV our enzyme provides higher cDNA yield, and has higher productivity on GC-rich RNA templates.

Benefits:

  • Effective for GC-rich RNA templates
  • Optimum working temperature is 55°С
  • High productivity

Area of applications:

  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR
  • cDNA synthesis for cloning
  • Generation of labelled cDNA probes for microarrays
  • RNA labelling
  • RNA analysis by primer extension

Storage and dilution buffer:

  • 20 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 100 mM KCl, 50% glycerol, 0.5% Tween-20, 1 mM TCEP

Concentration:

  • 200 units/μl (50-1000 units/μl upon request)

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 14 days at a temperature < +4°С

Additional information

Product Size

25 000 U/0.125 ml
50 000 U/0.25 ml
Free sample