RevM Revertase
80€ - 150€ *Net price
MMLV Reverse Transcriptase.
*B – supplied with 10X reverse transcription buffer.
*GF – supplied in glycerol-free buffer.
- Description
- Properties
- Additional information
- Documents
Description
RevM Revertase is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV) which performs the synthesis of a complementary strand of DNA (cDNA) on a single-stranded RNA template. Due to the presence of several mutations, the enzyme possesses inhibited RNase H activity and has increased thermostability at below 65°C, with an optimal temperature of 55°C. Compared to wild-type MMLV our enzyme provides higher cDNA yield, and has higher productivity on GC-rich RNA templates.
Benefits:
- Effective for GC-rich RNA templates
- Optimum working temperature is 55°С
- High productivity
Area of applications:
- First strand cDNA synthesis for RT-PCR and real-time RT-PCR
- cDNA synthesis for cloning
- Generation of labelled cDNA probes for microarrays
- RNA labelling
- RNA analysis by primer extension
Storage and dilution buffer:
- 20 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 100 mM KCl, 50% glycerol, 0.5% Tween-20, 1 mM TCEP
Concentration:
- 200 units/μl (50-1000 units/μl upon request)
Storage and transportation conditions:
- From -24°С to -16°С – 1 year
- From +2°С to +8°С – 14 days
Additional information
| Product Size | 50 000 U/0.25 ml/GF |
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