RevM Revertase

Description

RevM Revertase is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV) which performs the synthesis of a complementary strand of DNA (cDNA) on a single-stranded RNA template. Due to the presence of several mutations, the enzyme possesses inhibited RNase H activity and has increased thermostability at below 65°C, with an optimal temperature of 55°C. Compared to wild-type MMLV our enzyme provides higher cDNA yield, and has higher productivity on GC-rich RNA templates.

Benefits:

• Effective for GC-rich RNA templates
• Optimum working temperature is 55°С
• High productivity

Area of applications:

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• cDNA synthesis for cloning
• Generation of labelled cDNA probes for microarrays
• RNA labelling
• RNA analysis by primer extension

Storage and dilution buffer:

• 20 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 100 mM KCl, 50% glycerol, 0.5% Tween-20, 1 mM TCEP

Concentration:

• 200 units/μl (50-1000 units/μl upon request)

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From +2°С to +8°С – 14 days

Additional information