Phi29 DNA Polymerase

50 - 720 *Net price

Polymerase for multiple displacement DNA amplification.
Supplied with 10x reaction buffer.

Product code: E-PHI Category:
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Description

Phi29 DNA Polymerase is a highly processive enzyme with powerful strand displacement activity which useful for highly efficient isothermal DNA amplification. Phi29 DNA Polymerase also possesses a 3’→5’ exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA.

Benefits:

  • Isothermal amplification
  • Extreme processivity
  • High fidelity

Area of applications:

  • Isothermal DNA amplification for sequencing or cloning
  • Rolling circle amplification (RCA)
  • Multiple displacement amplification (MDA)
  • Amplification of DNA for SNP and STR detection
  • Protein primed or RNA primed DNA amplification

Concentration:

  • 10 units/μl (5-50 units/μl upon request)

Storage and dilution buffer:

  • 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 100 mM KCl, 0.5% Tween-20, 2 mM TCEP, 50% glycerol

Storage and transportation conditions:

  • From -24°С to -16°С – 1 year

Additional information

Product Size

5 000 U/0.5 ml/B
1 000 U/0.1 ml/B
250 U/0.025 ml/B
Free sample

Certificate of Analysis