Phi29 DNA Polymerase

Description

Phi29 DNA Polymerase is a highly processive enzyme with powerful strand displacement activity which useful for highly efficient isothermal DNA amplification. Phi29 DNA Polymerase also possesses a 3’→5’ exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA.

Benefits:

• Isothermal amplification
• Extreme processivity
• High fidelity

Area of applications:

• Rolling circle amplification (RCA): generation of periodic DNA nanotemplates
• Multiple displacement amplification (MDA)
• Amplification of DNA for SNP and STR detection
• Pathogenic organisms or metagenomes
• DNA template preparation for sequencing

Concentration:

• 10 units/μl (5-50 units/μl upon request)

Storage and dilution buffer:

• 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM TCEP, 100 mM KCl, 0.5% Tween-20, 50% glycerol

Storage and transportation conditions:

• From -24°С to -16°С – 2 years

Additional information