Phi29 DNA Polymerase

50 - 720 *Net price

Polymerase for multiple displacement DNA amplification.
Supplied with 10x reaction buffer.

Product code: E-PHI Category:
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Phi29 DNA Polymerase is a highly processive enzyme with powerful strand displacement activity which useful for highly efficient isothermal DNA amplification. Phi29 DNA Polymerase also possesses a 3’→5’ exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA.


  • Isothermal amplification
  • Extreme processivity
  • High fidelity

Area of applications:

  • Rolling circle amplification (RCA): generation of periodic DNA nanotemplates
  • Multiple displacement amplification (MDA)
  • Amplification of DNA for SNP and STR detection
  • Pathogenic organisms or metagenomes
  • DNA template preparation for sequencing


  • 10 units/μl (5-50 units/μl upon request)

Storage and dilution buffer:

  • 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM TCEP, 100 mM KCl, 0.5% Tween-20, 50% glycerol

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years

Additional information

Product Size

250 U/0.025 ml
1 000 U/0.1 ml
5 000 U/0.5 ml
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