Diamant TaqAF DNA Polymerase

Description

Diamant TaqAF DNA Polymerase is a recombinant Taq polymerase with dual aptamer/chemical inactivation. Diamant TaqAF DNA Polymerase has a high inactivation level and shows no activity after its incubation for 1 hour at 60°С. Enzyme is activated by 15 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during the PCR setup. Additional aptameric inactivation increased reproducibility in diagnostic applications and stability under storage.
The enzyme catalyses the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction and also exhibits 5’ exonuclease activity.

Benefits:

• High inactivation level
• High activity at the end of PCR
• Suitable for one-step RT-PCR
• Suitable for PCR with pre-amplification
• Increased stability under storage
• Reversible hot-start

Area of applications:

• Multiplex PCR
• Routine PCR
• Low copy PCR
• PCR with dual-labelled probes
• PCR with intercalating dyes
• PCR with pre-amplification

Storage and dilution buffer:

• 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

• 5 units/μl (20 units/μl for glycerol-free enzyme)

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From +2°С to +8°С – 14 days

Additional information