Diamant TaqAD DNA Polymerase

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Hot-start Taq DNA polymerase with dual antibody/aptamer inactivation.
Supplied with 10x PCR buffer and 50mM MgCl2.

Product code: E-TADP Category:
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Description

Diamant TaqAD DNA Polymerase is a high-quality hot-start polymerase with superior sensitivity and specificity compared to native Taq polymerase. The reagent is composed of a high-purified recombinant Taq DNA polymerase, specific low-molecular weight aptamer and antibodies which inhibits enzymatic activity of polymerase at room temperature. According to our tests, the polymerase keeps inactive during the incubation with a substrate for about 20 minutes at 55°C. Activation of the enzyme starts automatically during the first cycle of DNA denaturation. Due to aptamer the polymerase restores its re-inactivation at the end of PCR. Presence of two types of inhibitors makes it possible to use the enzyme in diagnostic RT-PCR kits with pre-amplification.

Benefits:

  • Amplification of PCR fragments up to 5 000 bp
  • Suitable for one-step RT-PCR
  • Suitable for test kits with pre-amplification
  • Reversible hot-start

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual-labelled probes
  • PCR with intercalating dyes

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

  • 5 units/μl (5-20 units/μl upon request)

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 14 days at a temperature < +25°С

Additional information

Product Size

1 000 U/0.2 ml
2 500 U/0.5 ml
25 000 U/5×1 ml
5 000 U/1 ml
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