Diamant TaqAD DNA Polymerase

Description

Diamant TaqAD DNA Polymerase is a high-quality hot-start polymerase with superior sensitivity and specificity compared to native Taq polymerase. The reagent is composed of a high-purified recombinant Taq DNA polymerase, specific low-molecular weight aptamer and antibodies which inhibits enzymatic activity of polymerase at room temperature. According to our tests, the polymerase keeps inactive during the incubation with a substrate for about 20 minutes at 55°C. Activation of the enzyme starts automatically during the first cycle of DNA denaturation. Due to aptamer the polymerase restores its re-inactivation at the end of PCR. Presence of two types of inhibitors makes it possible to use the enzyme in diagnostic RT-PCR kits with pre-amplification.

Benefits:

• Amplification of PCR fragments up to 5 000 bp
• Suitable for one-step RT-PCR
• Suitable for test kits with pre-amplification
• Reversible hot-start

Area of applications:

• Multiplex PCR
• Routine PCR
• Low copy PCR
• PCR with dual-labelled probes
• PCR with intercalating dyes

Storage and dilution buffer:

• 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

• 5 units/μl (20 units/μl for glycerol-free enzyme)

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From +2°С to +8°С – 14 days

Additional information