Blitz DNA Polymerase
180€ - 1 360€ *Net price
Chimeric polymerase with increased accuracy and resistance to inhibitors. Suitable for long range PCR and preparation of NGS libraries.
Supplied with 2.5X PCR buffer.
- Description
- Properties
- Additional information
- Documents
Description
Blitz DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The modifications of amino acid structure of the native Pfu results in shorter extension times (20 s/kb), more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Blitz a superior choice for cloning. This enzyme is suitable for all PCR applications requiring greater accuracy or long amplicons. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction and also exhibits 3’→5’ exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5’ exonuclease activity.
Benefits:
- Increased DNA synthesis rate (up to 3 000 bp/min)
- Long fragments amplification (10 kbp or longer)
- Increased inhibition resistance
Area of applications:
- PCR with increased synthesis accuracy
- Amplification of long DNA fragments
- Direct PCR without samples purifications
- NGS libraries preparation
Storage and dilution buffer:
- 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20
Concentration:
- 2 units/μl
Storage and transportation conditions:
- From -24°С to -16°С – 1 year
- From +2°С to +8°С – 14 days
Additional information
| Product Size | 5 000 U/2×1.25 ml/B |
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