RiboBlock H RNase Inhibitor

Description

RiboBlock H RNase Inhibitor is a 50 kDa recombinant protein of humane origin produced in Escherichia coli. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase and Reverse transcriptase. Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules that have complexed with ribonuclease. To prevent, the release of active ribonuclease, temperatures greater than 55°C and high concentrations of denaturing agents should be avoided. Due to genetic modification RiboBlock H RNase Inhibitor does not contain the pair of cysteines identified in the human native version that is very sensitive to oxidation, which causes inactivation of the inhibitor. As a result, RiboBlock H RNase inhibitor has significantly improved resistance to oxidation compared to the native human RNase inhibitor, and is stable at low DTT concentrations. This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).

Benefits:

• Inhibits ribonuclease activity of eukaryotic enzymes (RNase A, RNase B, RNase C)
• Increased stability
• Active over a wide pH range (pH 5-9)
• Stable at the presence of wide range PCR additives
• Improved resistance to oxidation
• Ideal for reactions where low DTT concentrations are required (e.g., qPCR, RT-PCR)

Area of applications:

• RNA isolation
• RT-PCR
• Synthesis of cDNA
• In vitro transcription and translation

Concentration:

• 40 units/μl
• 400 units/μl (for glycerol-free form)

Storage and transportation conditions:

• From -24°С to -16°С – 1 year
• From +2°С to +8°С – 14 days

Additional information