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5Х One-Step RT-PCR Mastermix is a ready-to-use universal reagent for the synthesis of cDNA on RNA matrix with the following polymerase chain reaction with real time identification of products (RT-PCR). Synthesis of cDNA and qPCR assay is carried out in one tube. The reagent provides reproducible results with high sensitivity (single copies of RNA) in RT-PCR.
5X One Step RT-PCR Mastermix contains all the necessary components for PCR assay, including: «warm start» reverse transcriptase, «hot start» thermostable DNA-polymerase, dNTPs and optimized buffer.
Due to some genetic modifications of MMLV everse transcriptase has inhibited RNase H activity, improved thermostability and increased productivity with GC-rich RNA matrix, resulting in the higher cDNA yield. «Hot-start» inactivated DNA-polymerase of the mastermix does not possess any enzymatic activity during PCR-mix preparation and reverse transcription that lead to significant increase of sensitivity and specificity of the assay. Special additives provide increased resistance to inhibitors.
- High sensitivity (single copies of RNA)
- Synthesis of cDNA and PCR is carried out in one tube
- Increased inhibition resistance
Area of applications:
- Multiplex RT-PCR
- Routine RT-PCR
- Low copy RT-PCR
- RT-PCR with dual-labeled probes
Storage and transportation conditions:
- From -24°С to -16°С – 2 years
- Up to 7 days at a temperature < +25°С