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5X One-Step RT-PCR Mastermix

250 - 3 600

Ready-to-use mix for RT-PCR. The reaction is carried out in one tube. Ideal solution for one-step detection of RNA viruses, including SARS-CoV-2

Kod produktu: M5-OS Kategoria:
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Opis

5Х One-Step RT-PCR Mastermix is a ready-to-use universal reagent for the synthesis of cDNA on RNA matrix with the following polymerase chain reaction with real time identification of products (RT-PCR). Synthesis of cDNA and qPCR assay is carried out in one tube. The reagent provides reproducible results with high sensitivity (single copies of RNA) in RT-PCR.

5X One Step RT-PCR Mastermix contains all the necessary components for PCR assay, including: «warm start» reverse transcriptase, «hot start» thermostable DNA-polymerase, dNTPs and optimized buffer.

Due to some genetic modifications of MMLV everse transcriptase has inhibited RNase H activity, improved thermostability and increased productivity with GC-rich RNA matrix, resulting in the higher cDNA yield. «Hot-start» inactivated DNA-polymerase of the mastermix does not possess any enzymatic activity during PCR-mix preparation and reverse transcription that lead to significant increase of sensitivity and specificity of the assay. Special additives provide increased resistance to inhibitors.

Benefits

  • High sensitivity (single copies of RNA)
  • Synthesis of cDNA and PCR is carried out in one tube
  • Increased inhibition resistance

Area of applications:

  • Multiplex RT-PCR
  • Routine RT-PCR
  • Low copy RT-PCR
  • RT-PCR with dual-labeled probes

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 7 days at a temperature < +25°С

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