5X Fast Dye qPCR U Mastermix

105 - 1 100

Ready-to-use mastermix for qPCR with intercalating dye, additionally contains UDG to prevent false positives results

Kod produktu: M5-FDU Kategoria:
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Opis

5Х Fast Dye probe qPCR U Mastermix is a ready-to-use reagent for real time polymerase chain reaction (qPCR). The reagent contains the following components: thermostable hot start DNA polymerase, dNTPs (including dUTP), uracil-DNA-glycosylase (UDG), intercalating dye, optimized buffer for PCR. The use of inactivated DNA polymerase, which does not possess enzymatic activity under the conditions of PCR mixture preparation, leads to an increase in the specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first cycle of DNA denaturation. The presence of dUTP in the reaction mixture leads to the fact that all amplification products will contain uracil. In this case, UDG effectively hydrolyzes uracil from single- and double-stranded DNA molecules during the preparation of the reaction, thus preventing the amplification of the products of previous PCR (amplicons). It is help to avoids false-positive results. UDG is completely inactivated during PCR and does not interfere with the amplification of the products of the current reaction. Special additives provide increased resistance to inhibitors. Mastermix can also contain reference dye ROX.

Benefits

  • High sensitivity (single copies of DNA)
  • Increased inhibition resistance
  • Prevention of false-positive results
  • Stable at temperatures of + 2°C – + 8°C

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with intercalating dye

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • From + 2°С to + 8°С – 14 months
  • Up to 14 days at a temperature < +25°С

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