Diamant TaqAD DNA Polymerase

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Hot start Taq DNA polymerase with activation temperature above 60°C

Kod produktu: E-TADP Kategoria:
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Opis

Diamant TaqAD DNA Polymerase is a high-quality hot-start polymerase with superior sensitivity and specificity compared to native Taq polymerase.
The reagent is composed of a high-purified recombinant Taq DNA polymerase, specific low-molecular weight aptamer and antibodies which inhibits enzymatic activity of polymerase at room temperature. According to our tests, the polymerase keeps inactive during the incubation with a substrate for about 1 hour at 60°C. Activation of the enzyme starts automatically during the first cycle of DNA denaturation.

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Free sample

Benefits:

  • Amplification of PCR fragments up to 5000 bp
  • Suitable for one-step RT-PCR
  • Suitable for test kits with preamplification
  • Reversible hot start

Area of applications:

  • Multiplex PCR
  • Routine PCR
  • Low copy PCR
  • PCR with dual labeled probes
  • PCR with intercalating dyes

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

  • 10 units/μl (5-20 units/μl upon request)

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 7 days at a temperature < +25°С