Blitz DNA Polymerase

180 - 1 360

Chimeric polymerase with increased accuracy and resistance to inhibitors. Suitable for preparation of NGS libraries. Supplied with 2.5X PCR buffer.

Kod produktu: E-BLP Kategoria:
Wyczyść
Zobacz koszyk
Zapytanie o rozmiar hurtowy

Opis

Blitz DNA Polymerase is a chimeric thermostable protein based on Pfu DNA polymerase containing DNA binding domain SSO7d. The enzyme has increased thermostability, processivity, accuracy and resistance to wide range of inhibitors.
Due to increased resistance to inhibitors the product can be used in «direct» PCR without preliminary purification of the samples (e.g. whole blood, saliva, urine etc). The enzyme is also tolerant to high ionic strength and dUTP. The reagent has an increased DNA synthesis rate (up to 4 kbp per minute) and capability of long fragments amplification (10 kbp or longer).

Informacje dodatkowe

Rozmiar produktu

Free sample

Benefits:

  • Increased DNA synthesis rate (up to 4000 bp/min)
  • Long fragments amplification (10 kbp or longer)
  • Increased inhibition resistance

Area of applications:

  • PCR with increased synthesis accuracy
  • Amplification of long DNA fragments
  • Direct PCR without samples purifications
  • NGS libraries preparation

Storage and dilution buffer:

  • 20 mМ Tris-HCl (pH 8.0), 0.5 mМ EDTA, 100 mМ KCl, 50% glycerol, 0.5% Tween-20

Concentration:

  • 2 units/μl

Storage and transportation conditions:

  • From -24°С to -16°С – 2 years
  • Up to 7 days at a temperature < +25°С